Heterokaryosis and you can parasexual recombination from inside the pathogenic stresses away from Fusarium oxysponrm
V. Heterokaryosis and you will parasexuality
Use the “0”spot for one of the biological parents and you can mention the stress matter on plate. Use the template towards the replicator. Incubate dos-3 days. Replicate the new segregants towards a number of shot dishes using a replicator with, e.g., 21 needles. Mark the newest dishes that have a number. Incubate 2-3 days. Get the exam plates and you can checklist new phenotypes regarding scoring desk. Just be sure to dictate the ploidy of one’s colonies into the base from the fresh indicators. Take a look at ploidy of uncertain colonies. Generate a list of the latest genotypes (you need a software application). Influence the latest part of brand new recombinants with the additional markers. And therefore markers are linked? Is it possible you get a hold of intrachromosomal recombination? In which linkage class ‘s the unfamiliar marker?
Inside check out i influence the fresh new gene buy and area out of the fresh centromere within the linkage class VI ofA. niger.Certain tips for your choice of mitotic recombinants are utilized. Read More
